| Charlotte County Sheriff and Punta Gorda Police | Charlotte | Public Safety | 49 |
| Orange County and Orlando Fire and EMS | Orange | Public Safety | 35 |
| Sarasota County Sheriff, Venice and North Port Police | Sarasota | Public Safety | 33 |
| Bay County Law Enforcement | Bay | Public Safety | 31 |
| Manatee County Sheriff | Manatee | Public Safety | 28 |
| Manatee County Fire and EMS | Manatee | Public Safety | 21 |
| Sarasota County Fire | Sarasota | Public Safety | 20 |
| Indian River County Sheriff | Indian River | Public Safety | 18 |
| Volusia County Fire and EMS | Volusia | Public Safety | 18 |
| Pinellas County Sheriff and EMS, Clearwater / St. Petersburg Police | Pinellas | Public Safety | 16 |
| Pinellas County Fire and EMS | Pinellas | Public Safety | 16 |
| Polk County Fire Rescue, Fire and EMS | Polk | Public Safety | 16 |
| Broward County Sheriff Dispatch - North | Broward | Public Safety | 15 |
| Clearwater Police - East | Pinellas | Public Safety | 12 |
| Gainesville Police and Alachua Fire | Alachua | Public Safety | 11 |
| Osceola County Public Safety | Osceola | Public Safety | 11 |
| Lee County and Cape Coral Fire Dispatch | Lee | Public Safety | 10 |
| Coral Springs and Parkland Public Safety | Broward | Public Safety | 8 |
| Broward County Fire/Rescue | Broward | Public Safety | 8 |
| Charlotte County Fire, Punta Gorda City Fire, and Punta Gorda Airport Fire | Charlotte | Public Safety | 8 |
| Collier County Fire and EMS Dispatch | Collier | Public Safety | 8 |
| Miami-Dade Police and Fire - Hammocks Dispatch and Central Dispatch | Miami-Dade | Public Safety | 8 |
| Miami-Dade County Fire Rescue North & East | Miami-Dade | Public Safety | 8 |
| Key West Public Safety | Monroe | Public Safety | 7 |
| City of Orlando Fire - Tac 1 and Tac 3 | Orange | Public Safety | 7 |
| Saint Johns County Fire / Rescue | St. Johns | Public Safety | 7 |
| DeSoto and Hardee Counties Fire Rescue | DeSoto | Public Safety | 6 |
| Port Canaveral Marine Channels 12 and 16 | Brevard | Marine | 4 |
| Miami-Dade Fire Rescue Tactical Channels | Miami-Dade | Public Safety | 4 |
| Levy County Public Safety | Levy | Public Safety | 4 |
| Seminole County Fire | Seminole | Public Safety | 4 |
| Bay County Fire/EMS | Bay | Public Safety | 3 |
| Broward County Police and Sheriff | Broward | Public Safety | 3 |
| Plantation Fire Department | Broward | Public Safety | 3 |
| Citrus County Fire | Citrus | Public Safety | 3 |
| Clay Fire Rescue | Clay | Public Safety | 3 |
| Lake City Fire | Columbia | Public Safety | 3 |
| Miami-Dade County Municipal Police Departments Dispatch | Miami-Dade | Public Safety | 3 |
| Miami Fire Rescue | Miami-Dade | Public Safety | 3 |
| FEC Bowden Yard | Duval | Rail | 3 |
| Hillsborough County Public Safety | Hillsborough | Public Safety | 3 |
| Sarasota South County Fire Departments | Sarasota | Public Safety | 3 |
| Seminole County Fire and EMS | Seminole | Public Safety | 3 |
| Baker County Fire Rescue | Baker | Public Safety | 2 |
| Brevard County Amateur Repeaters | Brevard | Amateur Radio | 2 |
| Indian Harbor Beach Fire | Brevard | Public Safety | 2 |
| CDARIN - The Caribbean Digital Amateur Radio International Network | Broward | Amateur Radio | 2 |
| Pompano Beach Fire/Rescue/BSO (East & West) | Broward | Public Safety | 2 |
| Broward County Fire/Rescue Dispatch | Broward | Public Safety | 2 |
| Liberty and Calhoun Counties Fire and EMS | Calhoun | Public Safety | 2 |
| Jackson County Fire | Jackson | Public Safety | 2 |
| Boca Raton Fire Rescue | Palm Beach | Public Safety | 2 |
| CSX S-Line | Sumter | Rail | 2 |
| Indian Harbor Beach Operations | Brevard | Public Safety | 1 |
| CSX and SFRTA Fort Lauderdale | Broward | Rail | 1 |
| City of Miami Police Dispatch - North | Miami-Dade | Public Safety | 1 |
| Pensacola Fire Department | Escambia | Public Safety | 1 |
| Mt Pleasant Fire | Gadsden | Public Safety | 1 |
| Sycamore Fire | Gadsden | Public Safety | 1 |
| Highland View Fire | Gulf | Public Safety | 1 |
| Howard Creek Fire | Gulf | Public Safety | 1 |
| Wewahitchka Fire | Gulf | Public Safety | 1 |
| TGIF DMR Talkgroup 31665 | Hernando | Amateur Radio | 1 |
| Miccosukee Volunteer Fire Rescue | Leon | Public Safety | 1 |
| CSX and Norfolk Southern - Callahan Area | Nassau | Rail | 1 |
| Central Florida CSX, Sunrail, Florida Central & Brightline/Florida East Coast | Orange | Rail | 1 |
| St Lucie County Amateur Radio Repeaters | St. Lucie | Amateur Radio | 1 |
| Munson Fire | Santa Rosa | Public Safety | 1 |
| Union County FR | Union | Public Safety | 1 |
| GARS Repeaters | Alachua | Amateur Radio | 0 |
| KC4NWK Repeater System | Alachua | Amateur Radio | 0 |
| Orlando Area NOAA Weather Radio KIH63 | Brevard | Other | 0 |
| N4TDX DMR TG 3124968 | Brevard | Amateur Radio | 0 |
| Fort Lauderdale Fire Rescue TAC D | Broward | Public Safety | 0 |
| Sumterville Area NOAA Weather Radio KPS505 | Citrus | Other | 0 |
| Palatka Area NOAA Weather Radio WNG522 | Clay | Other | 0 |
| Marco Island Police Dispatch | Collier | Public Safety | 0 |
| 14300 KHz Amateur Radio Nets | Collier | Amateur Radio | 0 |
| City of Miami Police Dispatch - South | Miami-Dade | Public Safety | 0 |
| City of Miami Police Dispatch - Central | Miami-Dade | Public Safety | 0 |
| Miami Dade Railroad Feed | Miami-Dade | Rail | 0 |
| Jacksonville Fire Rescue | Duval | Public Safety | 0 |
| CSX, FGAR & AGR Railroad - Pensacola Area | Escambia | Rail | 0 |
| Escambia County Area Marine | Escambia | Marine | 0 |
| Franklin County Fire Dispatch | Franklin | Public Safety | 0 |
| Carrabelle Volunteer Fire | Franklin | Public Safety | 0 |
| Concord Fire | Gadsden | Public Safety | 0 |
| Midway Fire | Gadsden | Public Safety | 0 |
| Greensboro Fire | Gadsden | Public Safety | 0 |
| Wetumpka Fire | Gadsden | Public Safety | 0 |
| Gretna Fire | Gadsden | Public Safety | 0 |
| Greenshade Dogtown | Gadsden | Public Safety | 0 |
| Hamilton County Dispatch | Hamilton | Public Safety | 0 |
| CSX Railroad Greater Valrico Area | Hillsborough | Rail | 0 |
| Tampa International Airport (KTPA) | Hillsborough | Aviation | 0 |
| Hillsborough County Sheriff's Office | Hillsborough | Public Safety | 0 |
| KV4SM Repeater | Hillsborough | Amateur Radio | 0 |
| Lee County Fire and EMS | Lee | Public Safety | 0 |
| Ft. Myers Amateur Radio Club Repeater | Lee | Amateur Radio | 0 |
| CSX and Florida Northern Railroads | Levy | Rail | 0 |